1x ht supplement (Thermo Fisher)
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Thermo Fisher
1x ht supplement
1x Ht Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ht/1x+hypoxanthine+thymidine++ht++supplement/pmc10409496-517-4-9
Average 90 stars, based on 1 article reviews
1x Ht Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ht/1x+hypoxanthine+thymidine++ht++supplement/pmc10409496-517-4-9
Average 90 stars, based on 1 article reviews
1x ht supplement - by Bioz Stars,
2026-10
90/100 stars
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Cloning:Article Title: Generation and characterization of human anti-human IL-21 neutralizing monoclonal antibodies Article Snippet: During fusion and the initial passages of fused cells, complete IMDM medium was applied with the addition of 1x HAT (hypoxanthine, aminopterin, thymidine; Mediatech #25-046-CI) for hybridoma selection. .. During the cloning process, cells were cultured with the addition of Cell Culture:Article Title: Generation and characterization of human anti-human IL-21 neutralizing monoclonal antibodies Article Snippet: During fusion and the initial passages of fused cells, complete IMDM medium was applied with the addition of 1x HAT (hypoxanthine, aminopterin, thymidine; Mediatech #25-046-CI) for hybridoma selection. .. During the cloning process, cells were cultured with the addition of Article Title: Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody Article Snippet: .. CHO DG44 cells were obtained as a gift from Dr. Yiannis Ioannou at the Department of Genetics and Genomic Sciences at the Icahn School of Medicine at Mount Sinai and were routinely cultured in Dulbecco’s Modified Eagle’s Medium (DMEM)/Ham’s F12 medium (Life Technologies/Gibco) supplemented with 10% fetal bovine serum (FBS, HyClone), 2mM L-glutamine, HAT Assay:Article Title: Generation and characterization of human anti-human IL-21 neutralizing monoclonal antibodies Article Snippet: During fusion and the initial passages of fused cells, complete IMDM medium was applied with the addition of 1x HAT (hypoxanthine, aminopterin, thymidine; Mediatech #25-046-CI) for hybridoma selection. .. During the cloning process, cells were cultured with the addition of Generated:Article Title: Mitochondrial dysfunction remodels one-carbon metabolism in human cells Article Snippet: Single cell colonies were analyzed for SHMT1 and SHMT2 expression by Western blotting; SHMT1 antibodies were Cell Signaling Tech (Danvers, MA) #12612, and SHMT2 antibodies were Thermo Scientific #PA5-32228. .. Double SHMT knockouts were generated likewise, except transfections were with 225 ng of each U6-sgRNA PCR product, and FACS sorted cells were grown in media further supplemented with Article Title: Mitochondrial dysfunction remodels one-carbon metabolism in human cells Article Snippet: Single cell colonies were analyzed for SHMT1 and SHMT2 expression by Western blotting; SHMT1 antibodies were Cell Signaling Tech (Danvers, MA) #12612, and SHMT2 antibodies were Thermo Scientific #PA5-32228. .. Double SHMT knockouts were generated likewise, except transfections were with 225 ng of each U6-sgRNA PCR product, and FACS sorted cells were grown in media further supplemented with Transfection:Article Title: Mitochondrial dysfunction remodels one-carbon metabolism in human cells Article Snippet: Single cell colonies were analyzed for SHMT1 and SHMT2 expression by Western blotting; SHMT1 antibodies were Cell Signaling Tech (Danvers, MA) #12612, and SHMT2 antibodies were Thermo Scientific #PA5-32228. .. Double SHMT knockouts were generated likewise, except transfections were with 225 ng of each U6-sgRNA PCR product, and FACS sorted cells were grown in media further supplemented with Article Title: Mitochondrial dysfunction remodels one-carbon metabolism in human cells Article Snippet: Single cell colonies were analyzed for SHMT1 and SHMT2 expression by Western blotting; SHMT1 antibodies were Cell Signaling Tech (Danvers, MA) #12612, and SHMT2 antibodies were Thermo Scientific #PA5-32228. .. Double SHMT knockouts were generated likewise, except transfections were with 225 ng of each U6-sgRNA PCR product, and FACS sorted cells were grown in media further supplemented with Polymerase Chain Reaction:Article Title: Mitochondrial dysfunction remodels one-carbon metabolism in human cells Article Snippet: Single cell colonies were analyzed for SHMT1 and SHMT2 expression by Western blotting; SHMT1 antibodies were Cell Signaling Tech (Danvers, MA) #12612, and SHMT2 antibodies were Thermo Scientific #PA5-32228. .. Double SHMT knockouts were generated likewise, except transfections were with 225 ng of each U6-sgRNA PCR product, and FACS sorted cells were grown in media further supplemented with Article Title: Mitochondrial dysfunction remodels one-carbon metabolism in human cells Article Snippet: Single cell colonies were analyzed for SHMT1 and SHMT2 expression by Western blotting; SHMT1 antibodies were Cell Signaling Tech (Danvers, MA) #12612, and SHMT2 antibodies were Thermo Scientific #PA5-32228. .. Double SHMT knockouts were generated likewise, except transfections were with 225 ng of each U6-sgRNA PCR product, and FACS sorted cells were grown in media further supplemented with FACS:Article Title: Mitochondrial dysfunction remodels one-carbon metabolism in human cells Article Snippet: Single cell colonies were analyzed for SHMT1 and SHMT2 expression by Western blotting; SHMT1 antibodies were Cell Signaling Tech (Danvers, MA) #12612, and SHMT2 antibodies were Thermo Scientific #PA5-32228. .. Double SHMT knockouts were generated likewise, except transfections were with 225 ng of each U6-sgRNA PCR product, and FACS sorted cells were grown in media further supplemented with Article Title: Mitochondrial dysfunction remodels one-carbon metabolism in human cells Article Snippet: Single cell colonies were analyzed for SHMT1 and SHMT2 expression by Western blotting; SHMT1 antibodies were Cell Signaling Tech (Danvers, MA) #12612, and SHMT2 antibodies were Thermo Scientific #PA5-32228. .. Double SHMT knockouts were generated likewise, except transfections were with 225 ng of each U6-sgRNA PCR product, and FACS sorted cells were grown in media further supplemented with Modification:Article Title: Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody Article Snippet: .. CHO DG44 cells were obtained as a gift from Dr. Yiannis Ioannou at the Department of Genetics and Genomic Sciences at the Icahn School of Medicine at Mount Sinai and were routinely cultured in Dulbecco’s Modified Eagle’s Medium (DMEM)/Ham’s F12 medium (Life Technologies/Gibco) supplemented with 10% fetal bovine serum (FBS, HyClone), 2mM L-glutamine, |